Induced movement of the magnetic beads and DNA-based dumbbell in a micro fluidic channel

B. Babić; R. Ghai; K. Dimitrov

doi:10.1117/12.753782

27 December 2007 Induced movement of the magnetic beads and DNA-based dumbbell in a micro fluidic channel

B. Babić, R. Ghai, K. Dimitrov

Proceedings Volume 6799, BioMEMS and Nanotechnology III; 67990U (2007) https://doi.org/10.1117/12.753782
Event: SPIE Microelectronics, MEMS, and Nanotechnology, 2007, Canberra, ACT, Australia

Abstract

We have explored controlled movement of magnetic beads and a dumbbell structure composed of DNA, a magnetic and a non-magnetic bead in a micro fluidic channel. Movement of the beads and dumbbells is simulated assuming that a net force is described as a superposition between the magnetic and hydrodynamic drag forces. Trajectories of beads and dumbbells are observed with optical light microscopy. The experimentally measured data show a good agreement with the simulations. This dynamical approach offers the prospect to stretch the DNA within the dumbbell and investigate its conformational changes. Further on, we demonstrate that short sonication can reduce multiple attachments of DNA to the beads.

Citation Download Citation

B. Babić, R. Ghai, and K. Dimitrov "Induced movement of the magnetic beads and DNA-based dumbbell in a micro fluidic channel", Proc. SPIE 6799, BioMEMS and Nanotechnology III, 67990U (27 December 2007); https://doi.org/10.1117/12.753782

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