Dynamic activation of Src induced by low-power laser irradiation in living cells mediated by reactive oxygen species

Juntao Zhang; Xuejuan Gao; Da Xing; Lei Liu

doi:10.1117/12.760229

11 January 2008 Dynamic activation of Src induced by low-power laser irradiation in living cells mediated by reactive oxygen species

Juntao Zhang, Xuejuan Gao, Da Xing, Lei Liu

Author Affiliations +

Proceedings Volume 6826, Optics in Health Care and Biomedical Optics III; 68260K (2008) https://doi.org/10.1117/12.760229
Event: Photonics Asia 2007, 2007, Beijing, China

Abstract

Low-power laser irradiation (LPLI) leads to photochemical reaction and then activates intracellular several signaling pathway. Reactive oxygen species (ROS) are considered to be the primary messengers produced by LPLI. Here, we studied the signaling pathway mediated by ROS upon the stimulation of LPLI. Src tyrosine kinases are well-known targets of ROS and can be activated by oxidative events. Using a Src reporter based on fluorescence resonance energy transfer (FRET) technique, we visualized the dynamic Src activation in Hela cells immediately after LPLI. Moreover, Src activity was enhanced by increasing the duration of LPLI. In addition, our results suggested that ROS were key mediators of Src activation, as ROS scavenger, vitamin C decreased and exogenous H₂O₂ increased the activity of Src. Meanwhile, Gö6983 loading did not block the effect of LPLI. CCK-8 experiments proved that cell vitality was prominently improved by LPLI with all the doses we applied in our experiments ranging from 3 to 25J/cm². The results indicated that LPLI/ROS/Src pathway may be involved in the LPLI biostimulation effects.

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Juntao Zhang, Xuejuan Gao, Da Xing, and Lei Liu "Dynamic activation of Src induced by low-power laser irradiation in living cells mediated by reactive oxygen species", Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 68260K (11 January 2008); https://doi.org/10.1117/12.760229

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