Fluorescence lifetime estimation of multiple near-infrared dyes in mice

Guobin Ma; Simon Fortier; Muriel Jean-Jacques; Niculae Mincu; Frederic Leblond; Zahia Ichalalene; Anader Benyamin-Seeyar; Mario Khayat

doi:10.1117/12.763544

12 February 2008 Fluorescence lifetime estimation of multiple near-infrared dyes in mice

Guobin Ma, Simon Fortier, Muriel Jean-Jacques, Niculae Mincu, Frederic Leblond, Zahia Ichalalene, Anader Benyamin-Seeyar, Mario Khayat

Author Affiliations +

Proceedings Volume 6850, Multimodal Biomedical Imaging III; 685003 (2008) https://doi.org/10.1117/12.763544
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

In order to precisely recover fluorescence lifetimes from bulk tissues, one needs to employ complex light propagation models (e.g., the radiative transfer equation or a simpler yet consistent approximation, the diffusion equation) requiring knowledge of the tissue optical properties. This can be computationally expensive and therefore not practical in many applications. We present a novel method to estimate the fluorescence lifetimes of multiple fluorophores embedded in mice. By assuming that the photon diffusion does not significantly change the fluorescence decay slope, the light propagation is simply modeled as a time-delay during lifetime estimation. Applications of this approach are demonstrated by simulation, phantom data, and in vivo experiments.

Citation Download Citation

Guobin Ma, Simon Fortier, Muriel Jean-Jacques, Niculae Mincu, Frederic Leblond, Zahia Ichalalene, Anader Benyamin-Seeyar, and Mario Khayat "Fluorescence lifetime estimation of multiple near-infrared dyes in mice", Proc. SPIE 6850, Multimodal Biomedical Imaging III, 685003 (12 February 2008); https://doi.org/10.1117/12.763544

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available