Relationship between light scattering and absorption due to cytochrome c oxidase reduction during loss of tissue viability in brains of rats

Satoko Kawauchi; Shunichi Sato; Hidetoshi Ooigawa; Hiroshi Nawashiro; Miya Ishihara; Makoto Kikuchi

doi:10.1117/12.762811

22 February 2008 Relationship between light scattering and absorption due to cytochrome c oxidase reduction during loss of tissue viability in brains of rats

Satoko Kawauchi, Shunichi Sato, Hidetoshi Ooigawa, Hiroshi Nawashiro, Miya Ishihara, Makoto Kikuchi

Author Affiliations +

Proceedings Volume 6864, Biomedical Applications of Light Scattering II; 68640K (2008) https://doi.org/10.1117/12.762811
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We performed simultaneous measurement of light scattering and absorption due to reduction of cytochrome c oxidase as intrinsic optical signals that are related to morphological characteristics and energy metabolism, respectively, for rat brains after oxygen/glucose deprivation by saline infusion. To detect change in light scattering, we determined the wavelength that was the most insensitive to change in light absorption due to the reduction of cytochrome c oxidase on the basis of multiwavelength analysis of diffuse reflectance data set for each rat. Then the relationships between scattering signal and absorption signals related to the reductions of heme aa₃ (605 nm) and CuA (830 nm) in cytochrome c oxidase were examined. Measurements showed that after starting saline infusion, the reduction of heme aa₃ started first; thereafter triphasic, large scattering change occurred (200-300 s), during which the reduction of CuA started. Despite such complex behaviors of IOSs, almost linear correlations were seen between the scattering signal and the heme aa₃-related absorption signal, while a relatively large animal-to-animal variation was observed in the correlation between the scattering signal and CuA-related absorption signal. Transmission electron microscopic observation revealed that dendritic swelling and mitochondrial deformation occurred in the cortical surface tissue after the triphasic scattering change. These results suggest that mitochondrial energy failure accompanies morphological alteration in the brain tissue and results in change in light scattering; light scattering will become an important indicator of tissue viability in brain.

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Satoko Kawauchi, Shunichi Sato, Hidetoshi Ooigawa, Hiroshi Nawashiro, Miya Ishihara, and Makoto Kikuchi "Relationship between light scattering and absorption due to cytochrome c oxidase reduction during loss of tissue viability in brains of rats", Proc. SPIE 6864, Biomedical Applications of Light Scattering II, 68640K (22 February 2008); https://doi.org/10.1117/12.762811

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KEYWORDS

Light scattering

Brain

Absorption

Scattering

Tissue optics

Tissues

Diffuse reflectance spectroscopy

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