Validation of nonlinear interferometric vibrational imaging as a molecular OCT technique by the use of Raman microscopy

Wladimir A. Benalcazar; Zhi Jiang; Daniel L. Marks; Joseph B. Geddes; Stephen A. Boppart

doi:10.1117/12.809384

19 February 2009 Validation of nonlinear interferometric vibrational imaging as a molecular OCT technique by the use of Raman microscopy

Wladimir A. Benalcazar, Zhi Jiang, Daniel L. Marks, Joseph B. Geddes, Stephen A. Boppart

Author Affiliations +

Proceedings Volume 7168, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIII; 71680T (2009) https://doi.org/10.1117/12.809384
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

We validate a molecular imaging technique called Nonlinear Interferometric Vibrational Imaging (NIVI) by comparing vibrational spectra with those acquired from Raman microscopy. This broadband coherent anti-Stokes Raman scattering (CARS) technique uses heterodyne detection and OCT acquisition and design principles to interfere a CARS signal generated by a sample with a local oscillator signal generated separately by a four-wave mixing process. These are mixed and demodulated by spectral interferometry. Its confocal configuration allows the acquisition of 3D images based on endogenous molecular signatures. Images from both phantom and mammary tissues have been acquired by this instrument and its spectrum is compared with its spontaneous Raman signatures.

Citation Download Citation

Wladimir A. Benalcazar, Zhi Jiang, Daniel L. Marks, Joseph B. Geddes, and Stephen A. Boppart "Validation of nonlinear interferometric vibrational imaging as a molecular OCT technique by the use of Raman microscopy", Proc. SPIE 7168, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIII, 71680T (19 February 2009); https://doi.org/10.1117/12.809384

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