Priyaveena Puvanakrishnan,1 Jaesook Park,1 Parameshwaran Diagaradjane,2 Jon A. Schwartz,3 Chris L. Coleman,3 Kelly L. Gill-Sharp,3 Kristina L. Sang,3 J. Donald Payne,3 Sunil Krishnan,2 James W. Tunnell1
1The Univ. of Texas at Austin (United States) 2The Univ. of Texas M.D. Anderson Cancer Ctr. (United States) 3Nanospectra Biosciences, Inc. (United States)
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Gold nanoshells (GNS) are a new class of nanoparticles that can be optically tuned to scatter or absorb light from the
near-ultraviolet to near-infrared (NIR) region by varying the core (dielectric silica) /shell (gold) ratio. In addition to
spectral tunability, GNS are inert and bioconjugatable making them potential labels for in vivo imaging and therapy of
tumors. We report the use of GNS as exogenous contrast agents for enhanced visualization of tumors using narrow band
imaging (NBI). NBI takes advantage of the strong NIR absorption of GNS to distinguish between blood and nanoshells
in the tumor by imaging in narrow wavelength bands in the visible and NIR, respectively. Using tissue-simulating
phantoms, we determined the optimum wavelengths to enhance contrast between blood and GNS. We then used the
optimum wavelengths for ex-vivo imaging of tumors extracted from human colon cancer xenograft bearing mice injected
with GNS. Systemically delivered GNS accumulated passively in tumor xenografts by the enhanced permeability and
retention (EPR) effect. Ex-vivo NBI of tumor xenografts demonstrated tumor specific heterogeneous distribution of GNS
with a clear distinction from the tumor vasculature. The results of this study demonstrate the feasibility of using GNS as
contrast agents to visualize tumors using NBI.
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Priyaveena Puvanakrishnan, Jaesook Park, Parameshwaran Diagaradjane, Jon A. Schwartz, Chris L. Coleman, Kelly L. Gill-Sharp, Kristina L. Sang, J. Donald Payne, Sunil Krishnan, James W. Tunnell, "Narrow band imaging of tumors using gold nanoshells," Proc. SPIE 7169, Advanced Biomedical and Clinical Diagnostic Systems VII, 716912 (23 February 2009); https://doi.org/10.1117/12.809613