Paper
12 February 2009 A high sensitivity multi-spectral three-dimensional fluorescence optical tomography system for small animal imaging
Changqing Li, Gregory S. Mitchell, Joyita Dutta, Sangtae Ahn, Richard M. Leahy, Simon R. Cherry
Author Affiliations +
Abstract
We have designed a three dimensional (3D) fluorescence optical tomography system for small animal imaging based on an innovative system geometry that uses a truncated conical mirror which permits the entire surface of the animal to be viewed simultaneously by a single CCD camera. Compared with traditional approaches that employ a flat mirror, the conical mirror system has approximately 3 times better measurement sensitivity. By utilizing a fast switching filter wheel (switching time < 100 milliseconds), emission data at multiple wavelengths can be efficiently collected. An array of appropriately shaped neutral density filters, mounted on a linear stage, can be used to increase the system measurement dynamic range by 3 orders of magnitude. An x-y galvo mirror scanning system makes it possible to scan a collimated laser beam to any location on the mouse surface. A pattern of structured light incident on the animal surface is used to extract the surface geometry. A finite element based algorithm is applied to model photon propagation in the turbid media and a preconditioned conjugate gradient (PCG) method is used to solve the large linear system matrix. The reconstruction algorithm and the system performance are evaluated by phantom experiments.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Changqing Li, Gregory S. Mitchell, Joyita Dutta, Sangtae Ahn, Richard M. Leahy, and Simon R. Cherry "A high sensitivity multi-spectral three-dimensional fluorescence optical tomography system for small animal imaging", Proc. SPIE 7174, Optical Tomography and Spectroscopy of Tissue VIII, 717415 (12 February 2009); https://doi.org/10.1117/12.809705
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KEYWORDS
Mirrors

Luminescence

Imaging systems

3D image processing

Optical tomography

3D image reconstruction

Optical filters

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