Cell palpation system for local mechanical properties of a cell with an optically manipulated particle

H. Miyoshi; T. Sugiura; K. Minato

doi:10.1117/12.809794

12 February 2009 Cell palpation system for local mechanical properties of a cell with an optically manipulated particle

H. Miyoshi, T. Sugiura, K. Minato

Author Affiliations +

Proceedings Volume 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII; 718211 (2009) https://doi.org/10.1117/12.809794
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

During cell adhesion and migration, a cell forms focal adhesion, which connects cytoskeleton with extracellular matrix (ECM) through integrin, and applies cytoskeletal force to the ECM through focal adhesion. In the initial phase of cell adhesion (initial adhesion), protein related to cell adhesion recruits other components to reinforce adhesion force and grows to focal complex. To study the mechanism of cell adhesion, we focused on relationship between variation of mechanical property of cell adhesion and related protein for cell adhesion. Especially, we approached by understanding mechanical property of initial adhesion. To measure this property, we developed a "cell palpation system", which utilizes optical tweezers to apply mechanical stimulus to a cell and to investigate reactive force. As below, this system gives information on the mechanical property (membrane support tension) and a time course of the property by using an optically manipulated microbead through an analysis based on mechanical model of this microbead. To create cell adhesion between the microbead and cell surface, the microbead was coated with collagen and we investigated the mechanical property of initial adhesion. And we analyzed the processes in relation to maturation of initial adhesion at a single molecular level.

Citation Download Citation

H. Miyoshi, T. Sugiura, and K. Minato "Cell palpation system for local mechanical properties of a cell with an optically manipulated particle", Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 718211 (12 February 2009); https://doi.org/10.1117/12.809794

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE