Continuous-flow PCR using segmented flow and integrating sample preparation

Holger Becker; Nadine Hlawatsch; Cornelia Carstens; Richard Klemm; Claudia Gärtner

doi:10.1117/12.809980

23 February 2009 Continuous-flow PCR using segmented flow and integrating sample preparation

Holger Becker, Nadine Hlawatsch, Cornelia Carstens, Richard Klemm, Claudia Gärtner

Proceedings Volume 7207, Microfluidics, BioMEMS, and Medical Microsystems VII; 72070J (2009) https://doi.org/10.1117/12.809980
Event: SPIE MOEMS-MEMS: Micro- and Nanofabrication, 2009, San Jose, California, United States

Abstract

Continuous-flow PCR has proven to be a powerful method for the amplification of genetic material due to its high speed and the possibility to perform amplicon detection and separation on-chip. A unique possibility of this method is the simultaneous amplification of several samples within a single chip by sample stacking, either having identical samples in several sample plugs separated by e.g. a mineral oil or using different samples in each sample plug. We have demonstrated the viability of sample stacking with a commercially available continuous-flow PCR system with a variety of protocols and samples. Further integration steps like thermal lysis and on-chip lyophilisate storage have been performed, with subsequent successful PCR. Chip modules for DNA extraction either with magnetic beads or membrane filters have been developed.

Citation Download Citation

Holger Becker, Nadine Hlawatsch, Cornelia Carstens, Richard Klemm, and Claudia Gärtner "Continuous-flow PCR using segmented flow and integrating sample preparation", Proc. SPIE 7207, Microfluidics, BioMEMS, and Medical Microsystems VII, 72070J (23 February 2009); https://doi.org/10.1117/12.809980

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