The character of biological tissue's SHG spectrum

Long Tian; Zhouyi Guo; Xiaoyuan Deng; Juan Zhai; Zhengfei Zhuang

doi:10.1117/12.843751

28 October 2009 The character of biological tissue's SHG spectrum

Long Tian, Zhouyi Guo, Xiaoyuan Deng, Juan Zhai, Zhengfei Zhuang

Author Affiliations +

Proceedings Volume 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009); 75191O (2009) https://doi.org/10.1117/12.843751
Event: Photonics and Optoelectronics Meetings 2009, 2009, Wuhan, China

Abstract

In biological tissue, the relative strongly SHG (second-harmonic Generation) will be shown in the collagen and the cell membrane with dye molecules under the irradiation of laser. The SHG has a broad prospect in detecting and imaging of the biological tissue for its non-phototoxicity and non-photobleaching. In biological tissue, not only the SHG intensity and emission angle will have more obvious change, but also the spectrum of the SHG will be subject to certain changes when the temperature in the outside world and its structure got a obviously change. According to Kuzyk and Kruhlak's dipole-free sum-over-states theory which gives a introduction for the nonlinear susceptibilities, the relationship between hyperpolarizability of biological tissue, environment temperature and biological tissue's structure is shown in mathematics. In the conditions of control the temperature in experiments, the biological tissue's structure shift can be detected by analyze the SHG spectrum of biological. Also diverse biological tissues' differences in structure can be demonstrated in the spectrum. The change of SHG spectrum for the same biological tissue with environment temperature is discussed. Therefore, SHG spectroscopy analysis provides a new technology for the process of biological tissue lesions. Beside, this research gives a theory results provided by environment temperature which give an explanation for experiment result.

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Long Tian, Zhouyi Guo, Xiaoyuan Deng, Juan Zhai, and Zhengfei Zhuang "The character of biological tissue's SHG spectrum", Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 75191O (28 October 2009); https://doi.org/10.1117/12.843751

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KEYWORDS

Second-harmonic generation

Tissues

Magnesium

Biological research

Collagen

Molecules

Analytical research

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