In vivo dual-modality imaging of lymphatic systems using indocyanine green in rats: three-dimensional photoacoustic imaging and planar fluorescence imaging

Chulhong Kim; Kwang Hyun Song; Lihong V. Wang

doi:10.1117/12.841964

23 February 2010 In vivo dual-modality imaging of lymphatic systems using indocyanine green in rats: three-dimensional photoacoustic imaging and planar fluorescence imaging

Chulhong Kim, Kwang Hyun Song, Lihong V. Wang

Author Affiliations +

Proceedings Volume 7564, Photons Plus Ultrasound: Imaging and Sensing 2010; 75642W (2010) https://doi.org/10.1117/12.841964
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

The purpose of this study is to map non-invasively sentinel lymph nodes (SLNs) and lymphatic vessels of rats in vivo using FDA-approved indocyanine green (ICG) and two non-ionizing imaging modalities: volumetric spectroscopic photoacoustic (PA) imaging, which measures optical absorption, and planar fluorescence imaging, which measures fluorescent emission. SLNs and lymphatic vessels were clearly visible after a 0.2 ml-intradermal-injection of 1 mM ICG in both imaging systems. We also imaged deeply positioned lymph nodes in vivo by layering biological tissues on top of rats. These two modalities, when used together with ICG, have the potential to map SLNs in axillary staging and to study tumor metastasis in breast cancer patients.

Citation Download Citation

Chulhong Kim, Kwang Hyun Song, and Lihong V. Wang "In vivo dual-modality imaging of lymphatic systems using indocyanine green in rats: three-dimensional photoacoustic imaging and planar fluorescence imaging", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75642W (23 February 2010); https://doi.org/10.1117/12.841964

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