Optical-resolution photoacoustic microscopy of amyloid-&#946; deposits in vivo

Song Hu; Ping Yan; Konstantin Maslov; Jin-Moo Lee; Lihong V. Wang

doi:10.1117/12.843919

23 February 2010 Optical-resolution photoacoustic microscopy of amyloid-β deposits in vivo

Song Hu, Ping Yan, Konstantin Maslov, Jin-Moo Lee, Lihong V. Wang

Author Affiliations +

Proceedings Volume 7564, Photons Plus Ultrasound: Imaging and Sensing 2010; 75643D (2010) https://doi.org/10.1117/12.843919
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Advances in high-resolution imaging have permitted microscopic observations within the brains of living animals. Applied to Alzheimer's disease (AD) mouse models, multiphoton microscopy has opened a new window to study the real-time appearance and growth of amyloid plaques. Here, we report an alternative technology-optical-resolution photoacoustic microscopy (OR-PAM)-for in vivo imaging of amyloid plaques in a transgenic AD mouse model. In vivo validation using multiphoton microscopy shows that OR-PAM has sufficient sensitivity and spatial resolution to identify amyloid plaques in living brains. In addition, with dual-wavelength OR-PAM, the three-dimensional morphology of amyloid plaques and the surrounding microvasculature are imaged simultaneously through a cranial window. In vivo transcranial OR-PAM imaging of amyloid plaques is highly likely once the imaging parameters are optimized.

Citation Download Citation

Song Hu, Ping Yan, Konstantin Maslov, Jin-Moo Lee, and Lihong V. Wang "Optical-resolution photoacoustic microscopy of amyloid-β deposits in vivo", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75643D (23 February 2010); https://doi.org/10.1117/12.843919

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