Confocal fluorescence detection of cell-based assays using a digital micromirror device

Jong-ryul Choi; Jong Hwan Sung; Michael L. Shuler; Donghyun Kim

doi:10.1117/12.841738

10 February 2010 Confocal fluorescence detection of cell-based assays using a digital micromirror device

Jong-ryul Choi, Jong Hwan Sung, Michael L. Shuler, Donghyun Kim

Proceedings Volume 7596, Emerging Digital Micromirror Device Based Systems and Applications II; 759608 (2010) https://doi.org/10.1117/12.841738
Event: SPIE MOEMS-MEMS, 2010, San Francisco, California, United States

Abstract

We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (φ = 10 μm) as an equivalent to a cell.

Citation Download Citation

Jong-ryul Choi, Jong Hwan Sung, Michael L. Shuler, and Donghyun Kim "Confocal fluorescence detection of cell-based assays using a digital micromirror device", Proc. SPIE 7596, Emerging Digital Micromirror Device Based Systems and Applications II, 759608 (10 February 2010); https://doi.org/10.1117/12.841738

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