Single cell electroporation using proton beam fabricated biochips

S. Homhuan; B. Zhang; F.-S. Sheu; A. A. Bettiol; F. Watt

doi:10.1117/12.850657

13 May 2010 Single cell electroporation using proton beam fabricated biochips

S. Homhuan, B. Zhang, F.-S. Sheu, A. A. Bettiol, F. Watt

Proceedings Volume 7716, Micro-Optics 2010; 77160V (2010) https://doi.org/10.1117/12.850657
Event: SPIE Photonics Europe, 2010, Brussels, Belgium

Abstract

We report the design and fabrication of a novel single cell electroporation biochip fabricated by the Proton Beam Writing technique (PBW), a new technique capable of direct-writing high-aspect-ratio nano and microstructures. The biochip features nickel micro-electrodes with straight-side walls between which individual cells are positioned. By applying electrical impulses across the electrodes, SYTOX® Green nucleic acid stain is incorporated into mouse neuroblastoma (N2a) cells. When the stain binds with DNA inside the cell nucleus, green fluorescence is observed upon excitation from a halogen lamp. Three parameters; electric field strength, pulse duration, and the number of pulses have been considered and optimized for the single cell electroporation. The results show that our biochip gives successfully electroporated cells . This single cell electroporation system represents a promising method for investigating the introduction of a wide variety of fluorophores, nanoparticles, quantum dots, DNAs and proteins into cells.

Citation Download Citation

S. Homhuan, B. Zhang, F.-S. Sheu, A. A. Bettiol, and F. Watt "Single cell electroporation using proton beam fabricated biochips", Proc. SPIE 7716, Micro-Optics 2010, 77160V (13 May 2010); https://doi.org/10.1117/12.850657

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available