Study on suitable for regeneration system of genetic transformation of kiwifruit

Yun Xiang Yuan

doi:10.1117/12.887367

4 February 2011 Study on suitable for regeneration system of genetic transformation of kiwifruit

Yun Xiang Yuan

Proceedings Volume 7752, PIAGENG 2010: Photonics and Imaging for Agricultural Engineering; 775206 (2011) https://doi.org/10.1117/12.887367
Event: International Conference on Photonics and Image in Agricultural Engineering (PIAGENG 2010), 2010, Qingdao, China

Abstract

The stems of Actinidia Qinmei were taken as explants and induced callus formation after having gained the aseptic seedling in primary culture successfully, and then the calli were placed on different combinations regeneration medium. The results showed that the induction medium added 1 mg/L 6-BA and 0.1 mg/L NAA was beneficial to the callus induction in dark culture condition. The suitable regeneration medium was MS containing 2.0 mg/L 6BA, 0.1 mg/L NAA and 3% (W/V) sucrose, it could improve obviously the frequency of regenerated shoots. This method separated the callus induction from shoot regeneration and obtained more callus to optimize the regenerated medium, and also was advantageous to Kiwifruit genetic transformation.

Citation Download Citation

Yun Xiang Yuan "Study on suitable for regeneration system of genetic transformation of kiwifruit", Proc. SPIE 7752, PIAGENG 2010: Photonics and Imaging for Agricultural Engineering, 775206 (4 February 2011); https://doi.org/10.1117/12.887367

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