A processing technology of two-photon microscopic image of nasopharyngeal cancer cells

Hongxin Lin; Tingyin Wang; Rong Chen; Yanan Wu; Shangyuan Feng; Zufang Huang; Jinping Lei; Lin Ou; Guannan Chen; Yongzeng Li

doi:10.1117/12.869806

8 November 2010 A processing technology of two-photon microscopic image of nasopharyngeal cancer cells

Hongxin Lin, Tingyin Wang, Rong Chen, Yanan Wu, Shangyuan Feng, Zufang Huang, Jinping Lei, Lin Ou, Guannan Chen, Yongzeng Li

Author Affiliations +

Proceedings Volume 7845, Optics in Health Care and Biomedical Optics IV; 784529 (2010) https://doi.org/10.1117/12.869806
Event: Photonics Asia 2010, 2010, Beijing, China

Abstract

Nasopharyngeal carcinoma (NPC) is one of the most common malignancies in china, with a deep and hidden localization. Recently, methods for early diagnosis of NPC has become one of the most important research topics in medical field. Early monitoring of morphological change of NPC cells during the carcinogenesis is of great importance, and early information extracted from the NPC cells during the initial stage of NPC is critical for diagnosis and treatment. In this paper, image processing methods for two-photon microscopic image of NPC cells was investigated with the purpose of providing useful information for early diagnosis and treatment of NPC. There is abundant information in a two-photon microscopic image of NPC cells, which can be analyzed and processed by means of computer and image pattern processing algorithm. In this paper, firstly, a mathematical method of transform of Bottom-hat based on Matlab platform was employed to enhance the image of NPC cells, making the image easier to distinguish; Then, several classical edge detection algorithms were compared and discussed, for example, Roberts operator, Prewitt operator, and Canny operator etc. According to the inherent characteristics of two-photon microscopic image of NPC cells, corrosion algorithm was used to define the edge of NPC cells. Furthermore, the article gets the iterative threshold segmentation after noise denoising, on the other hand, improved discriminant analysis was adopted for threshold segmentation of NPC cells, better results were obtained.

Citation Download Citation

Hongxin Lin, Tingyin Wang, Rong Chen, Yanan Wu, Shangyuan Feng, Zufang Huang, Jinping Lei, Lin Ou, Guannan Chen, and Yongzeng Li "A processing technology of two-photon microscopic image of nasopharyngeal cancer cells", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 784529 (8 November 2010); https://doi.org/10.1117/12.869806

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Image segmentation

Image processing

Image enhancement

Edge detection

Image processing algorithms and systems

Cancer

MATLAB

Show All Keywords

Keywords/Phrases

Search In:

Publication Years