Two-photon autofluorescence spectroscopy of oral mucosa tissue

Kert Edward; Tuya Shilagard; Suimin Qiu; Gracie Vargas

doi:10.1117/12.875049

28 February 2011 Two-photon autofluorescence spectroscopy of oral mucosa tissue

Kert Edward, Tuya Shilagard, Suimin Qiu, Gracie Vargas

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 79031Q (2011) https://doi.org/10.1117/12.875049
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

The survival rate for individuals diagnosed with oral cancer is correlated with the stage of detection. Thus the development of novel techniques for the earliest possible detection of malignancies is of critical importance. Single photon (1P) autofluorescence spectroscopy has proven to be a powerful diagnostic tool in this regard, but 2P (two photon) spectroscopy remains essentially unexplored. In this investigation, a spectroscopic system was incorporated into a custom-built 2P laser scanning microscope. Oral cancer was induced in the buccal pouch of Syrian Golden hamsters by tri-weekly topical application of 9,10-dimethyl-1,2-benzanthracene (DMBA).Three separated sites where investigated in each hamster at four excitation wavelengths from 780 nm to 890 nm. A Total of 8 hamsters were investigated (4 normal and 4 DMBA treated). All investigated sites were imaged via 2p imaging, marked for biopsy, processed for histology and H&E staining, and graded by a pathologist. The in vivo emission spectrum for normal, mild/high grade dysplasia and squamous cell carcinoma is presented. It is shown that the hamsters with various stages of dysplasia are characterized by spectral differences as a function of depth and excitation wavelength, compared to normal hamsters.

Citation Download Citation

Kert Edward, Tuya Shilagard, Suimin Qiu, and Gracie Vargas "Two-photon autofluorescence spectroscopy of oral mucosa tissue", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031Q (28 February 2011); https://doi.org/10.1117/12.875049

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