Detecting metastasis of gastric carcinoma using high-resolution micro-CT system: in vivo small animal study

Junting Liu; Jie Tian; Jimin Liang; Xiangsi Li; Xiang Yang; Xiaofeng Chen; Yi Chen; Yuanfang Zhou; Xiaorui Wang

doi:10.1117/12.878410

9 March 2011 Detecting metastasis of gastric carcinoma using high-resolution micro-CT system: in vivo small animal study

Junting Liu, Jie Tian, Jimin Liang, Xiangsi Li, Xiang Yang, Xiaofeng Chen, Yi Chen, Yuanfang Zhou, Xiaorui Wang

Author Affiliations +

Proceedings Volume 7965, Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging; 79650L (2011) https://doi.org/10.1117/12.878410
Event: SPIE Medical Imaging, 2011, Lake Buena Vista (Orlando), Florida, United States

Abstract

Immunocytochemical and immunofluorescence staining are used for identifying the characteristics of metastasis in traditional ways. Micro-computed tomography (micro-CT) is a useful tool for monitoring and longitudinal imaging of tumor in small animal in vivo. In present study, we evaluated the feasibility of the detection for metastasis of gastric carcinoma by high-resolution micro-CT system with omnipaque accumulative enhancement method in the organs. Firstly, a high-resolution micro-CT ZKKS-MCT-sharp micro-CT was developed by our research group and Guangzhou Zhongke Kaisheng Medical Technology Co., Ltd. Secondly, several gastric carcinoma models were established through inoculating 2x10⁶ BGC-823 gastric carcinoma cells subcutaneously. Thirdly, micro-CT scanning was performed after accumulative enhancement method of intraperitoneal injection of omnipaque contrast agent containing 360 mg iodine with a concentration of 350 mg I/ml. Finally, we obtained high-resolution anatomical information of the metastasis in vivo in a BALB/c NuNu nude mouse, the 3D tumor architecture is revealed in exquisite detail at about 35 μm spatial resolution. In addition, the accurate shape and volume of the micrometastasis as small as 0.78 mm³ can be calculated with our software. Overall, our data suggest that this imaging approach and system could be used to enhance the understanding of tumor proliferation, metastasis and could be the basis for evaluating anti-tumor therapies.

Citation Download Citation

Junting Liu, Jie Tian, Jimin Liang, Xiangsi Li, Xiang Yang, Xiaofeng Chen, Yi Chen, Yuanfang Zhou, and Xiaorui Wang "Detecting metastasis of gastric carcinoma using high-resolution micro-CT system: in vivo small animal study", Proc. SPIE 7965, Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging, 79650L (9 March 2011); https://doi.org/10.1117/12.878410

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KEYWORDS

In vivo imaging

Tumors

Imaging systems

Reconstruction algorithms

Kidney

Magnesium

Tumor growth modeling

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