Optical-digital restoration of out-of-focus color images detected in microscopy

R. Ortiz-Sosa; L. R. Berriel-Valdos; J. F. Aguilar; J. Carranza-Gallardo; I. J. Orlando-Guerrero; S. Mejía-Romero

doi:10.1117/12.903416

2 November 2011 Optical-digital restoration of out-of-focus color images detected in microscopy

R. Ortiz-Sosa, L. R. Berriel-Valdos, J. F. Aguilar, J. Carranza-Gallardo, I. J. Orlando-Guerrero, S. Mejía-Romero

Proceedings Volume 8011, 22nd Congress of the International Commission for Optics: Light for the Development of the World; 801184 (2011) https://doi.org/10.1117/12.903416
Event: International Commission for Optics (ICO 22), 2011, Puebla, Mexico

Abstract

An optical-digital method is proposed for restoring trichromatic images out-of-focus, detected with a monochromatic CCD. The optical system (OS) is a microscope working in bright field mode and the source of illumination is polychromatic. To carry out the restoration, an estimated of the intensity point spread function is obtained from the image of a non-resolved object (quasi-point source) by the OS in three wavelengths. From the image of the quasi-point source, its spectrum is calculated for obtaining the optical transfer function associated with each wavelength. The Wiener filter is built with the spectrum, and the restoration is made for each color. By an addition of the three restored images, an estimated of the trichromatic object is recovered. The obtained results and conclusions are presented.

Citation Download Citation

R. Ortiz-Sosa, L. R. Berriel-Valdos, J. F. Aguilar, J. Carranza-Gallardo, I. J. Orlando-Guerrero, and S. Mejía-Romero "Optical-digital restoration of out-of-focus color images detected in microscopy", Proc. SPIE 8011, 22nd Congress of the International Commission for Optics: Light for the Development of the World, 801184 (2 November 2011); https://doi.org/10.1117/12.903416

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