In vivo N-acetyl cysteine reduce hepatocyte death by induced acetaminophen

Chih-Ju Lin; Feng-Chieh Li; Sheng-Shun Wang; Hsuan-Shu Lee; Chen-Yuan Dong

doi:10.1117/12.889621

2 June 2011 In vivo N-acetyl cysteine reduce hepatocyte death by induced acetaminophen

Chih-Ju Lin, Feng-Chieh Li, Sheng-Shun Wang, Hsuan-Shu Lee, Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 8092, Medical Laser Applications and Laser-Tissue Interactions V; 80921L (2011) https://doi.org/10.1117/12.889621
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

Acetaminophen (APAP) is the famous drug in global, and taking overdose Acetaminophen will intake hepatic cell injure. Desptie substantial progress in our understanding of the mechanism of hepatocellular injury during the last 40 years, many aspects of the pathophysiology are still unknown or controversial.1 In this study, mice are injected APAP overdose to damage hepatocyte. APAP deplete glutathione and ATP of cell, N-Acetyl Cysteine (NAC) plays an important role to protect hepatocytes be injury. N-Acetyl Cysteine provides mitochondrial to produce glutathione to release drug effect hepatocyte. By 6-carboxyfluorescein diacetate (6-CFDA) metabolism in vivo, glutathione keep depleting to observe the hepatocyte morphology in time. Without NAC, cell necrosis increase to plasma membrane damage to release 6-CFDA, that's rupture. After 6-CFDA injection, fluorescence will be retained in hepatocyte. For cell retain with NAC and without NAC are almost the same. With NAC, the number of cell rupture decreases about 75%.

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Chih-Ju Lin, Feng-Chieh Li, Sheng-Shun Wang, Hsuan-Shu Lee, and Chen-Yuan Dong "In vivo N-acetyl cysteine reduce hepatocyte death by induced acetaminophen", Proc. SPIE 8092, Medical Laser Applications and Laser-Tissue Interactions V, 80921L (2 June 2011); https://doi.org/10.1117/12.889621

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KEYWORDS

Cell death

Injuries

Mode conditioning cables

In vivo imaging

Liver

Physics

Luminescence

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