Time resolved optical biopsy spectroscopy of normal, benign and malignant tissues from NADH and FAD changes

V. Masilamani; B. B. Das; J. Secor; M. AlSalhi; S. B. Amer; K. Farhat; D. Rabah; R. R. Alfano

doi:10.1117/12.906913

31 January 2012 Time resolved optical biopsy spectroscopy of normal, benign and malignant tissues from NADH and FAD changes

V. Masilamani, B. B. Das, J. Secor, M. AlSalhi, S. B. Amer, K. Farhat, D. Rabah, R. R. Alfano

Author Affiliations +

Proceedings Volume 8220, Optical Biopsy X; 822009 (2012) https://doi.org/10.1117/12.906913
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Histo pathological examination is the gold standard to discriminate between benign and malignant growth of tissue. But this is invasive and stressful. Hence many non invasive imaging techniques, such as CT, MRI, PET, etc are employed, each having certain advantages and disadvantages. In this context optical biopsy is a newly emerging technique, since it employs non-ionizing radiation like light or laser, which could be shined directly or launched through optical fiber to reach any part of the body. This paper reports results of time resolved emission spectra of 24 excised tissue sample (normal control=12; benign=4; malignant=8) of breast and prostate, employing a 390nm, 100 fs, Ti-Sapphire laser pulses. The fluorescence decay times were measured using streak camera and fitted for single and bi- exponential decays with reliability of 97%. Our results show the distinct difference between normal, benign and malignant tissues attributed changes of NADH and FAD levels.

Citation Download Citation

V. Masilamani, B. B. Das, J. Secor, M. AlSalhi, S. B. Amer, K. Farhat, D. Rabah, and R. R. Alfano "Time resolved optical biopsy spectroscopy of normal, benign and malignant tissues from NADH and FAD changes", Proc. SPIE 8220, Optical Biopsy X, 822009 (31 January 2012); https://doi.org/10.1117/12.906913

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