A Raman spectroscopic approach for the cultivation-free identification of microbes

Petra Rösch; Stephan Stöckel; Susann Meisel; Ute Münchberg; Sandra Kloß; Dragana Kusic; Wilm Schumacher; Jürgen Popp

doi:10.1117/12.901241

29 November 2011 A Raman spectroscopic approach for the cultivation-free identification of microbes

Petra Rösch, Stephan Stöckel, Susann Meisel, Ute Münchberg, Sandra Kloß, Dragana Kusic, Wilm Schumacher, Jürgen Popp

Proceedings Volume 8311, Optical Sensors and Biophotonics III; 83111B (2011) https://doi.org/10.1117/12.901241
Event: SPIE/OSA/IEEE Asia Communications and Photonics, 2011, Shanghai, China

Abstract

In the last years the identification of microorganisms by means of different IR and Raman spectroscopic techniques has become quite popular. Most of the studies however apply the various vibrational spectroscopic methods to bulk samples which require at least a short cultivation time of several hours. Nevertheless, bulk identification methods achieve high classification rates which enable even the discrimination between closely related strains or the distinction between resistance capabilities. However, applying micro-Raman spectroscopy with visible excitation wavelengths enables for the detection of single microorganisms. Especially for time critical process like the fast diagnosis of severe diseases or the identification of bacterial contamination on food samples or pharmaceuticals, a cultivation-free identification of bacteria is required. In doing so, we established different isolation techniques in combination with Raman spectroscopic identification. Isolating bacteria from different matrixes always has an impact on the Raman spectroscopic identification capability. Therefore, these isolation techniques have to be specially designed to fulfill the spectroscopic requirements. In total the method should enable the identification of pathogens within the first 3 hours.

Citation Download Citation

Petra Rösch, Stephan Stöckel, Susann Meisel, Ute Münchberg, Sandra Kloß, Dragana Kusic, Wilm Schumacher, and Jürgen Popp "A Raman spectroscopic approach for the cultivation-free identification of microbes", Proc. SPIE 8311, Optical Sensors and Biophotonics III, 83111B (29 November 2011); https://doi.org/10.1117/12.901241

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