Picosecond-resolved FRET on non-amplified DNA for identifying individuals genetically susceptible to type-1 diabetes

Luca Nardo; Giovanna Tosi; Maria Bondani; Roberto Accolla; Alessandra Andreoni

doi:10.1117/12.919182

22 May 2012 Picosecond-resolved FRET on non-amplified DNA for identifying individuals genetically susceptible to type-1 diabetes

Luca Nardo, Giovanna Tosi, Maria Bondani, Roberto Accolla, Alessandra Andreoni

Author Affiliations +

Proceedings Volume 8375, Advanced Photon Counting Techniques VI; 837512 (2012) https://doi.org/10.1117/12.919182
Event: SPIE Defense, Security, and Sensing, 2012, Baltimore, Maryland, United States

Abstract

By tens-of-picosecond resolved fluorescence detection we study Förster resonance energy transfer between a donor and a black-hole-quencher bound at the 5'- and 3'-positions of an oligonucleotide probe matching the highly polymorphic region between codons 51 and 58 of the human leukocyte antigen DQB1 0201 allele, conferring susceptibility to type-1 diabetes. The probe is annealed with non-amplified genomic DNAs carrying either the 0201 sequence or other DQB1 allelic variants. We detect the longest-lived donor fluorescence in the case of hybridization with the 0201 allele and definitely faster and distinct decays for the other allelic variants, some of which are single-nucleotide polymorphic.

Citation Download Citation

Luca Nardo, Giovanna Tosi, Maria Bondani, Roberto Accolla, and Alessandra Andreoni "Picosecond-resolved FRET on non-amplified DNA for identifying individuals genetically susceptible to type-1 diabetes", Proc. SPIE 8375, Advanced Photon Counting Techniques VI, 837512 (22 May 2012); https://doi.org/10.1117/12.919182

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