DNA detection using molecular beacon in soft-glass microstructured optical fibers

Linh V. Nguyen; Stephen C. Warren-Smith; Alan Cooper; Tanya M. Monro

doi:10.1117/12.966847

7 November 2012 DNA detection using molecular beacon in soft-glass microstructured optical fibers

Linh V. Nguyen, Stephen C. Warren-Smith, Alan Cooper, Tanya M. Monro

Author Affiliations +

Proceedings Volume 8421, OFS2012 22nd International Conference on Optical Fiber Sensors; 84215M (2012) https://doi.org/10.1117/12.966847
Event: OFS2012 22nd International Conference on Optical Fiber Sensor, 2012, Beijing, China

Abstract

We report, for the first time to our knowledge, the detection of specific strands of DNA based on a soft-glass microstructured optical fiber (MOF) platform loaded with molecular beacons (MBs). This detection scheme brings together the intrinsic advantage of MOF for low-volume sensing with a label-free approach, and is highly specific in identifying complementary (cDNA) from non-complementary (nDNA) sequences at room temperature. The detection limit of the current sensing scheme is demonstrated to be improvable via coupling to different sets of guided core modes by means of longitudinal offset in coupling light from the source to the fiber.

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Linh V. Nguyen, Stephen C. Warren-Smith, Alan Cooper, and Tanya M. Monro "DNA detection using molecular beacon in soft-glass microstructured optical fibers", Proc. SPIE 8421, OFS2012 22nd International Conference on Optical Fiber Sensors, 84215M (7 November 2012); https://doi.org/10.1117/12.966847

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