Near infrared FRET using wide-field fluorescence lifetime imaging in live animals

Lingling Zhao; Ken Abe; Margarida Barroso; Xavier Intes

doi:10.1117/12.2033588

18 June 2013 Near infrared FRET using wide-field fluorescence lifetime imaging in live animals

Lingling Zhao, Ken Abe, Margarida Barroso, Xavier Intes

Proceedings Volume 8801, Novel Biophotonic Techniques and Applications II; 88010A (2013) https://doi.org/10.1117/12.2033588
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

One of the challenges in anti-cancer drug delivery systems is to quantitatively discriminate non-specific receptorindependent tumor accumulation from receptor-mediated uptake into the tumor cells. To overcome this challenge, we develop a new near infrared fluorescence resonance energy transfer fluorescence lifetime imaging (NIR FRET FLIM) technique with wide-field illumination strategies to validate and characterize cellular uptake in both cancer cells and normal cells with different donor-acceptor ratios in vitro and in vivo. Our results demonstrate that NIR FRET FLIM can quantitatively distinguish receptor-bound from unbound donor in live animals with high sensitivity and high accuracy. Thus, it has a great potential for the quantitative detection of targeted delivery systems for diagnostic and therapeutic use.

Citation Download Citation

Lingling Zhao, Ken Abe, Margarida Barroso, and Xavier Intes "Near infrared FRET using wide-field fluorescence lifetime imaging in live animals", Proc. SPIE 8801, Novel Biophotonic Techniques and Applications II, 88010A (18 June 2013); https://doi.org/10.1117/12.2033588

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