Multiphoton imaging of biological samples during freezing and heating

H. G. Breunig; A. Uchugonova; K. König

doi:10.1117/12.2038452

28 February 2014 Multiphoton imaging of biological samples during freezing and heating

H. G. Breunig, A. Uchugonova, K. König

Proceedings Volume 8948, Multiphoton Microscopy in the Biomedical Sciences XIV; 894819 (2014) https://doi.org/10.1117/12.2038452
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

We applied multiphoton microscopic imaging to observe freezing and heating effects in plant- and animal cell samples. The experimental setups consisted of a multiphoton imaging system and a heating and cooling stage which allows for precise temperature control from liquid nitrogen temperature (-196°C; 77 K) up to +600°C (873 K) with heating/freezing rates between 0.01 K/min and 150 K/min. Two multiphoton imaging systems were used: a system based on a modified optical microscope and a flexible mobile system. To illustrate the imaging capabilities, plant leafs as well as animal cells were microscopically imaged in vivo during freezing based on autofluorescence lifetime and intensity of intrinsic molecules. The measurements illustrate the usefulness of multiphoton imaging to investigate freezing effects on animal and plant cells.

Citation Download Citation

H. G. Breunig, A. Uchugonova, and K. König "Multiphoton imaging of biological samples during freezing and heating", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 894819 (28 February 2014); https://doi.org/10.1117/12.2038452

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KEYWORDS

Fluorescence lifetime imaging

Imaging systems

Luminescence

In vivo imaging

Temperature metrology

Liquids

Microscopes

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