Phase mask optimization for 3D parallax EDF microscopy

Ingeborg E. Beckers; Michael Gierlack; Robert Höppel; Jürgen Landskron

doi:10.1117/12.2036144

12 March 2014 Phase mask optimization for 3D parallax EDF microscopy

Ingeborg E. Beckers, Michael Gierlack, Robert Höppel, Jürgen Landskron

Proceedings Volume 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI; 89490Z (2014) https://doi.org/10.1117/12.2036144
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Extended depth-of-field (EDF) microscopy is a well-investigated and very simple method to obtain projection images with an extended depth of focus. Despite its advantages of being a real-time method applicable to any microscopic mode with high lateral resolution that can be simply realized by extending a commercial microscope, the lack of z-correlation is still a problem. In this work we present a combined technique of EDF and stereomicroscopy. By cross-correlation depth information is obtained. Finally, 3D images are reconstructed for best phase masks and simulation results are evaluated experimentally.

Citation Download Citation

Ingeborg E. Beckers, Michael Gierlack, Robert Höppel, and Jürgen Landskron "Phase mask optimization for 3D parallax EDF microscopy", Proc. SPIE 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, 89490Z (12 March 2014); https://doi.org/10.1117/12.2036144

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available