Intracellular light-induced release of signaling molecules from gold-coated liposomes

Gabriel V. Orsinger; Joshua D. Williams; Marek Romanowski

doi:10.1117/12.2040854

24 March 2014 Intracellular light-induced release of signaling molecules from gold-coated liposomes

Gabriel V. Orsinger, Joshua D. Williams, Marek Romanowski

Proceedings Volume 8955, Colloidal Nanoparticles for Biomedical Applications IX; 89551G (2014) https://doi.org/10.1117/12.2040854
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The combination of laser light and composite nanovesicles enables unique opportunities for precise delivery to, and ondemand release of molecular compounds within, single cells at high spatiotemporal resolution. Here, we demonstrate precise delivery and intracellular release of molecules from gold-coated liposomes via near infrared (NIR) light. The plasmon resonant gold shell provides a light-sensitive trigger for on-demand content release from thermosensitive liposomes. Two demonstrations of intracellular delivery and release from gold-coated liposomes are presented here. The first example uses microinjection to preload gold-coated liposomes into a single cell, followed by exposure to onresonant NIR laser light to trigger release of a fluorescent nuclear dye intracellularly. In the second delivery and release demonstration, gold-coated liposomes encapsulating inositol trisphosphate (IP3), a ubiquitous secondary messenger in cell signaling cascades, passively accumulate within cells via endocytosis. Exposure to on-resonant NIR laser wavelength of light induces rapid release of IP3 from the intracellular liposomes and subsequent activation of Ca²⁺ signaling at a single cell, monitored by changes in fluorescence intensity of a Ca ²⁺-sensitive dye.

Citation Download Citation

Gabriel V. Orsinger, Joshua D. Williams, and Marek Romanowski "Intracellular light-induced release of signaling molecules from gold-coated liposomes", Proc. SPIE 8955, Colloidal Nanoparticles for Biomedical Applications IX, 89551G (24 March 2014); https://doi.org/10.1117/12.2040854

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