Here we present a setup, which is based on two-photon microscopy and multi-dimensional time-correlated single-photoncounting (TCSPC). The presented method allows consecutive NADH and FAD imaging with high specificity and minimal side effects. In addition the setup is useful for simultaneous monitoring of NADH FLIM and intracellular oxygen partial pressure (pO2). With the two-channel FLIM/PLIM system we could show that pO2 is investigated simultaneously with different metabolic parameters in living cells. |
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CITATIONS
Cited by 1 scholarly publication.
Fluorescence lifetime imaging
Luminescence
Oxygen
Phosphorescence
Mode conditioning cables
Imaging systems
Microscopes