New insights and system designs for temporally focused multiphoton optogenetics

Tom Mayblum; Adi Schejter; Hod Dana; Shy Shoham

doi:10.1117/12.2078678

5 March 2015 New insights and system designs for temporally focused multiphoton optogenetics

Tom Mayblum, Adi Schejter, Hod Dana, Shy Shoham

Author Affiliations +

Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 932928 (2015) https://doi.org/10.1117/12.2078678
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Temporal focusing (TF) multiphoton systems constitute a powerful solution for cellular resolution optogenetic stimulation and recording in three-dimensional, scattering tissue. Here, we address two fundamental aspects in the design of such systems: first, we examine the design of TF systems with specific optical sectioning by comparatively analyzing previously published results. Next, we develop a solution for obtaining TF in a flexible three-dimensional pattern of cellmatched focal spots. Our solution employs spatio-temporal focusing (SSTF) in a unique optical system design that can be integrated before essentially any multiphoton imaging or stimulation system.

Citation Download Citation

Tom Mayblum, Adi Schejter, Hod Dana, and Shy Shoham "New insights and system designs for temporally focused multiphoton optogenetics", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 932928 (5 March 2015); https://doi.org/10.1117/12.2078678

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