Optimizing experimental conditions for stimulated emission depletion microscopy in biophotonics

Karl Beeson; Mary J. Potasek; Evgueni Parilov

doi:10.1117/12.2079479

13 March 2015 Optimizing experimental conditions for stimulated emission depletion microscopy in biophotonics

Karl Beeson, Mary J. Potasek, Evgueni Parilov

Proceedings Volume 9369, Photonic Instrumentation Engineering II; 93690V (2015) https://doi.org/10.1117/12.2079479
Event: SPIE OPTO, 2015, San Francisco, California, United States

Abstract

Using a novel numerical method we show how to optimize the resolution enhancement of stimulated emission depletion (STED) by simulating the entire process including the absorption, overlapping multiple beams and stimulated emission. We provide calculations showing that for fixed donut pulse energy, a longer donut pulse length can result in greater resolution enhancement than a shorter donut pulse length. These results show how it is possible to use our simulations to design the best experimental conditions for STED resolution enhancement and illustrate the importance of having a software program that includes both multiple beams and stimulated emission.

Citation Download Citation

Karl Beeson, Mary J. Potasek, and Evgueni Parilov "Optimizing experimental conditions for stimulated emission depletion microscopy in biophotonics", Proc. SPIE 9369, Photonic Instrumentation Engineering II, 93690V (13 March 2015); https://doi.org/10.1117/12.2079479

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