3D imaging of apoptosis by FRET, light sheet fluorescence and scattering microsopy

Herbert Schneckenburger; Petra Weber; Sarah Schickinger; Verena Richter; Thomas Bruns; Michael Wagner

doi:10.1117/12.2183607

15 July 2015 3D imaging of apoptosis by FRET, light sheet fluorescence and scattering microsopy

Herbert Schneckenburger, Petra Weber, Sarah Schickinger, Verena Richter, Thomas Bruns, Michael Wagner

Proceedings Volume 9537, Clinical and Biomedical Spectroscopy and Imaging IV; 953716 (2015) https://doi.org/10.1117/12.2183607
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany

Abstract

Non-radiative cell membrane associated energy transfer (FRET) from a cyan (ECFP) to a yellow (EYFP) fluorescent protein is used for detection of apoptosis in multi-cellular tumor spheroids. Low light exposure in light sheet fluorescence microscopy is combined with the detection of spectral changes and prolonged fluorescence lifetimes of the donor ECFP upon apoptosis. In view of future label-free detection preliminary experiments of light scattering microscopy with high angular resolution are reported which may give additional information on morphological changes.

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Herbert Schneckenburger, Petra Weber, Sarah Schickinger, Verena Richter, Thomas Bruns, and Michael Wagner "3D imaging of apoptosis by FRET, light sheet fluorescence and scattering microsopy", Proc. SPIE 9537, Clinical and Biomedical Spectroscopy and Imaging IV, 953716 (15 July 2015); https://doi.org/10.1117/12.2183607

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KEYWORDS

Luminescence

Cell death

Light scattering

Microscopy

Scattering

Fluorescence resonance energy transfer

Energy transfer

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