Low-temperature bonded glass-membrane microfluidic device for in vitro organ-on-a-chip cell culture models

Kyall J. Pocock; Xiaofang Gao; Chenxi Wang; Craig Priest; Clive A. Prestidge; Kazuma Mawatari; Takehiko Kitamori; Benjamin Thierry

doi:10.1117/12.2202461

22 December 2015 Low-temperature bonded glass-membrane microfluidic device for in vitro organ-on-a-chip cell culture models

Kyall J. Pocock, Xiaofang Gao, Chenxi Wang, Craig Priest, Clive A. Prestidge, Kazuma Mawatari, Takehiko Kitamori, Benjamin Thierry

Author Affiliations +

Proceedings Volume 9668, Micro+Nano Materials, Devices, and Systems; 96680W (2015) https://doi.org/10.1117/12.2202461
Event: SPIE Micro+Nano Materials, Devices, and Applications, 2015, Sydney, New South Wales, Australia

Abstract

The integration of microfluidics with living biological systems has paved the way to the exciting concept of “organson- a-chip”, which aims at the development of advanced in vitro models that replicate the key features of human organs. Glass based devices have long been utilised in the field of microfluidics but the integration of alternative functional elements within multi-layered glass microdevices, such as polymeric membranes, remains a challenge.
To this end, we have extended a previously reported approach for the low-temperature bonding of glass devices that enables the integration of a functional polycarbonate porous membrane. The process was initially developed and optimised on specialty low-temperature bonding equipment (μTAS2001, Bondtech, Japan) and subsequently adapted to more widely accessible hot embosser units (EVG520HE Hot Embosser, EVG, Austria). The key aspect of this method is the use of low temperatures compatible with polymeric membranes. Compared to borosilicate glass bonding (650 °C) and quartz/fused silica bonding (1050 °C) processes, this method maintains the integrity and functionality of the membrane (T_g 150 °C for polycarbonate). Leak tests performed showed no damage or loss of integrity of the membrane for up to 150 hours, indicating sufficient bond strength for long term cell culture. A feasibility study confirmed the growth of dense and functional monolayers of Caco-2 cells within 5 days.

Citation Download Citation

Kyall J. Pocock, Xiaofang Gao, Chenxi Wang, Craig Priest, Clive A. Prestidge, Kazuma Mawatari, Takehiko Kitamori, and Benjamin Thierry "Low-temperature bonded glass-membrane microfluidic device for in vitro organ-on-a-chip cell culture models", Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems, 96680W (22 December 2015); https://doi.org/10.1117/12.2202461

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