3D imaging of the cleared intact murine colon with light sheet microscopy

B. Zufiria; D. I. Bocancea; M. V. Gómez-Gaviro; J. J. Vaquero; M. Desco; M. Fresno; J. Ripoll; A. Arranz

doi:10.1117/12.2212039

9 March 2016 3D imaging of the cleared intact murine colon with light sheet microscopy

B. Zufiria, D. I. Bocancea, M. V. Gómez-Gaviro, J. J. Vaquero, M. Desco, M. Fresno, J. Ripoll, A. Arranz

Proceedings Volume 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII; 97130Q (2016) https://doi.org/10.1117/12.2212039
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We here show 3D light sheet microscopy images of fixed and cleared murine colon tissue in-toto, which offer relevant cellular information without the need for physically sectioning the tissue. We have applied the recently developed CUBIC protocol (Susaki et al. Cell 157:726, 2014) for colon tissues and have found that this clearing protocol enables imaging all the way to the central part of the lumen with cellular resolution, thus opening new ways for 3D imaging of colon samples.

Citation Download Citation

B. Zufiria, D. I. Bocancea, M. V. Gómez-Gaviro, J. J. Vaquero, M. Desco, M. Fresno, J. Ripoll, and A. Arranz "3D imaging of the cleared intact murine colon with light sheet microscopy", Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 97130Q (9 March 2016); https://doi.org/10.1117/12.2212039

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