Fluorescence intensity enhancement mechanism in presence of plasmonic nanoparticles

Sumana Das; Brahmanandam Javvaji; Krishna Harika Villa; Akshata Arikady; Gopalkrishna M. Hegde; D. Roy Mahapatra

doi:10.1117/12.2217940

22 April 2016 Fluorescence intensity enhancement mechanism in presence of plasmonic nanoparticles

Sumana Das, Brahmanandam Javvaji, Krishna Harika Villa, Akshata Arikady, Gopalkrishna M. Hegde, D. Roy Mahapatra

Proceedings Volume 9724, Plasmonics in Biology and Medicine XIII; 97240K (2016) https://doi.org/10.1117/12.2217940
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Plasmonic nanoparticles have several applications ranging from catalysis to super-resolution imaging and information storage. Maximum density of optical states is confined on the nanoparticle surface, which are collectively excited by electromagnetic wave and are called surface plasmons. Using nanoparticle based plasmonic interaction with biological cells in an optical fiber integrated microfluidic chip, we show enhancement of fluorescence intensity. Signal from in situ imaging is analyzed with various controls to understand the mechanism. The present study is focused on nanoparticle interaction with cells and on optimization strategies to maximize the fluorescence enhancement at the vicinity of the nanoparticles, for important applications such as fluorescence-based biochip platforms. Result is also correlated ZnO nanoparticle effect on fluorescence enhancement, which has different optoelectronic properties compared to gold nanoparticles. Electromagnetic wave field model is employed to simulate the effect of gold and ZnO nanoparticle on cell with the assumption that the nanoparticles are a collection of discrete dipoles, which are ordered with the fluorescence molecules on cell wall. Simulation model shows enhancement of fluorescence intensity is occurred in presence of gold nanoparticles rather than ZnO nanoparticles, which is confirmed with experimental data.

Citation Download Citation

Sumana Das, Brahmanandam Javvaji, Krishna Harika Villa, Akshata Arikady, Gopalkrishna M. Hegde, and D. Roy Mahapatra "Fluorescence intensity enhancement mechanism in presence of plasmonic nanoparticles", Proc. SPIE 9724, Plasmonics in Biology and Medicine XIII, 97240K (22 April 2016); https://doi.org/10.1117/12.2217940

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KEYWORDS

Nanoparticles

Luminescence

Zinc oxide

Plasmonics

Gold

Plasmons

Electromagnetic radiation

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