Electrically biased GaAs/AlGaAs heterostructures for enhanced detection of bacteria

Mohammad R. Aziziyan; Walid M. Hassen; Jan J. Dubowski

doi:10.1117/12.2219856

23 March 2016 Electrically biased GaAs/AlGaAs heterostructures for enhanced detection of bacteria

Mohammad R. Aziziyan, Walid M. Hassen, Jan J. Dubowski

Author Affiliations +

Proceedings Volume 9737, Synthesis and Photonics of Nanoscale Materials XIII; 97370E (2016) https://doi.org/10.1117/12.2219856
Event: SPIE LASE, 2016, San Francisco, California, United States

Abstract

We have examined the influence of electrical bias on immobilization of bacteria on the surface of GaAs/AlGaAs heterostructures, functionalized with an alkanethiol based architecture. A mixture of biotinylated polyethylene glycol (PEG) thiol and hexadecanethiol was applied to attach neutravidin and antibodies targeting specific immobilization of Legionella pneumophila. An electrochemical setup was designed to bias biofunctionalized samples with the potential measured versus silver/silver chloride reference electrode in a three electrode configuration system. The immobilization efficiency has been examined with fluorescence microscopy after tagging captured bacteria with fluorescein labeled antibodies. We demonstrate more than 2 times enhanced capture of Legionella pneumophila, suggesting the potential of electrically biased biochips to deliver enhanced sensitivity in detecting these bacteria.

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Mohammad R. Aziziyan, Walid M. Hassen, and Jan J. Dubowski "Electrically biased GaAs/AlGaAs heterostructures for enhanced detection of bacteria", Proc. SPIE 9737, Synthesis and Photonics of Nanoscale Materials XIII, 97370E (23 March 2016); https://doi.org/10.1117/12.2219856

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