Quantification of two forms of green sulfur bacteria in their natural habitat using bacteriochlorophyll fluorescence spectra

Anastasia V. Kharcheva; Anna A. Zhiltsova; Olga N. Lunina; Alexander S. Savvichev; Svetlana V. Patsaeva

doi:10.1117/12.2229848

21 April 2016 Quantification of two forms of green sulfur bacteria in their natural habitat using bacteriochlorophyll fluorescence spectra

Anastasia V. Kharcheva, Anna A. Zhiltsova, Olga N. Lunina, Alexander S. Savvichev, Svetlana V. Patsaeva

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Proceedings Volume 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS); 99170P (2016) https://doi.org/10.1117/12.2229848
Event: Saratov Fall Meeting 2015, 2015, Saratov, Russian Federation

Abstract

Detection of phototropic organisms in their natural habitat using optical instruments operating under water is urgently needed for many tasks of ecological monitoring. While fluorescence methods are widely applied nowadays to detect and characterize phytoplankton communities, the techniques for detection and recognition of anoxygenic phototrophs are considered challenging. Differentiation of the forms of anoxygenic green sulfur bacteria in natural water using spectral techniques remains problematic. Green sulfur bacteria could be found in two forms, green-colored (containing BChl d in pigment compound) and brown-colored (containing BChl e), have the special ecological niche in such reservoirs. Separate determination of these microorganisms by spectral methods is complicated because of similarity of spectral characteristics of their pigments. We describe the novel technique of quantification of two forms of green sulfur bacteria directly in water using bacteriochlorophyll fluorescence without pigment extraction. This technique is noninvasive and could be applied in remote mode in the water bodies with restricted water circulation to determine simultaneously concentrations of two forms of green sulfur bacteria in their natural habitat.

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Anastasia V. Kharcheva, Anna A. Zhiltsova, Olga N. Lunina, Alexander S. Savvichev, and Svetlana V. Patsaeva "Quantification of two forms of green sulfur bacteria in their natural habitat using bacteriochlorophyll fluorescence spectra", Proc. SPIE 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 99170P (21 April 2016); https://doi.org/10.1117/12.2229848

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