Directional optical coherence tomography: a novel method to detect the changes in melanin pigmentation of the retinal pigment epithelium

Ratheesh K. Meleppat; Robert J. Zawadzki

doi:10.1117/12.2610289

7 March 2022 Directional optical coherence tomography: a novel method to detect the changes in melanin pigmentation of the retinal pigment epithelium

Ratheesh K. Meleppat, Robert J. Zawadzki

Author Affiliations +

Proceedings Volume PC11941, Ophthalmic Technologies XXXII; PC119410L (2022) https://doi.org/10.1117/12.2610289
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

A directional optical coherence tomography (OCT) was implemented to access both amplitude and sensitivity (directionality) of directional backscattering of the retinal pigment epithelium (RPE) cells. Intensity and directionality of RPE scattering from different mouse strains with varied melanin pigmentation levels (heavily pigmented, moderately pigmented agouti, and melanin-lacking albino mice) are measured and compared. Amplitude of RPE scattering is directly proportional to melanosome concentration, whereas the directionality is inversely related to the concentration of RPE melanosome. The diagnostic capability of this approach was illustrated using a mouse model of Stargardt disease (Abca4-/-), where a reduced melanin-pigmentation relative to wild-type control was detected..

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Ratheesh K. Meleppat and Robert J. Zawadzki "Directional optical coherence tomography: a novel method to detect the changes in melanin pigmentation of the retinal pigment epithelium", Proc. SPIE PC11941, Ophthalmic Technologies XXXII, PC119410L (7 March 2022); https://doi.org/10.1117/12.2610289

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