High-information-throughput metabolic imaging by mid-infrared microscopy coupled with vibrational probes

Lixue Shi; Wei Min

doi:10.1117/12.2607421

7 March 2022 High-information-throughput metabolic imaging by mid-infrared microscopy coupled with vibrational probes

Lixue Shi, Wei Min

Author Affiliations +

Proceedings Volume PC11957, Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry; PC119570J (2022) https://doi.org/10.1117/12.2607421
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Understanding metabolism is indispensable in unraveling the mechanistic basis of biological processes. However, in situ metabolic imaging tools are still lacking. Here we introduce a framework for mid-infrared (MIR) metabolic imaging by coupling the emerging high-information-throughput MIR microscopy with specifically designed IR-active vibrational probes. We present three categories of small vibrational tags including azide bond, 13C-edited carbonyl bond and deuterium-labeled probes to interrogate various metabolic activities in cells, small organisms and mice. Our technique is uniquely suited to metabolic imaging with high information throughput. In particular, we performed single-cell metabolic profiling and large-area metabolic imaging at tissue or organ level.

Conference Presentation

Citation Download Citation

Lixue Shi and Wei Min "High-information-throughput metabolic imaging by mid-infrared microscopy coupled with vibrational probes", Proc. SPIE PC11957, Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry, PC119570J (7 March 2022); https://doi.org/10.1117/12.2607421

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