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We demonstrate volumetric phase contrast imaging by using optical coherence tomography (OCT). In general, the randomness of the scatterers’ distribution prohibits the volumetric measurement of a meaningful phase in a scattering mode. Our method uses complex numerical manipulation of an en-face complex OCT and gives a transversally differential phase image similar to a differential interference contrast microscope (DIC). Not like the DIC, our method can arbitrarily select the amount and direction of the shear after the OCT acquisition. In addition it provides DIC-like images at arbitrary depths. This method is validated by using a 840-nm spectral domain OCT system. A zebrafish sample is measured over a 1-mm × 1-mm transversal scanning range.
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