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2 March 2022 Dynamic tomography by six-pack holography
Simcha K. Mirsky, Natan T. Shaked
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Proceedings Volume PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022; PC119720V (2022) https://doi.org/10.1117/12.2608135
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Typically, tomographic reconstruction of dynamic 3D samples is limited by sample movement speed and illumination scanning speed. However, using the six-pack holography technique that we have developed, 6 off-axis holograms of different illumination angles can be acquired in a single camera exposure, without sacrificing individual image resolution or field of view, and without requiring sample sparsity. Using these 6 holograms we reconstruct the 3D refractive index tomogram of a dynamic sample from each video frame using the optical diffraction tomography algorithm. We demonstrate our technique experimentally on flowing microbeads and swimming microalgae.
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Simcha K. Mirsky and Natan T. Shaked "Dynamic tomography by six-pack holography", Proc. SPIE PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, PC119720V (2 March 2022); https://doi.org/10.1117/12.2608135
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KEYWORDS
Tomography
Holography
Cameras
Holograms
Optical tomography
Flow cytometry
Image resolution
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