Cell disorder strength and cellular stiffness analysis across quantitative phase imaging systems

Cindy X. Chen; Albert Rancu; Steven M. Parker; Hillel Price; Adam Wax

doi:10.1117/12.2609229

2 March 2022 Cell disorder strength and cellular stiffness analysis across quantitative phase imaging systems

Cindy X. Chen, Albert Rancu, Steven M. Parker, Hillel Price, Adam Wax

Proceedings Volume PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022; PC119720Z (2022) https://doi.org/10.1117/12.2609229
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Disorder strength is a metric that is used to measure cell stiffness. Through calculating disorder strength from cell phase images reconstructed from various QPI systems, we have discovered that this parameter differs by system used for acquisition. With our model, we demonstrate that disorder strength is highly dependent on image resolution. When corrected with our simulation model, disorder strength measurements from different QPI systems can be directly compared and enable cross-system analysis. Cell disorder strength infers important biophysical information on cellular stiffness of normal cells, carcinogen-exposed cells, and cancer cells.

Conference Presentation

Citation Download Citation

Cindy X. Chen, Albert Rancu, Steven M. Parker, Hillel Price, and Adam Wax "Cell disorder strength and cellular stiffness analysis across quantitative phase imaging systems", Proc. SPIE PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, PC119720Z (2 March 2022); https://doi.org/10.1117/12.2609229

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