High resolution imaging of collagen architecture alterations in ovarian cancer and idiopathic pulmonary fibrosis

Paul J. Campagnola; Darian James; Emily Shelton; Nathan Sandbo; Manish Patankar

doi:10.1117/12.2636163

3 October 2022 High resolution imaging of collagen architecture alterations in ovarian cancer and idiopathic pulmonary fibrosis

Paul J. Campagnola, Darian James, Emily Shelton, Nathan Sandbo, Manish Patankar

Author Affiliations +

Proceedings Volume PC12228, Ultrafast Nonlinear Imaging and Spectroscopy X; PC122280B (2022) https://doi.org/10.1117/12.2636163
Event: SPIE Optical Engineering + Applications, 2022, San Diego, California, United States

Abstract

We have developed Second Harmonic Generation (SHG) microscope tools to selectively and specifically probe all levels of collagen architecture organization in human ovarian cancer and idiopathic pulmonary fibrosis. We classified normal and diseased tissues in both diseases based on the respective fiber morphology using a novel form of 3D machine learning. We also developed polarization sensitive SHG methods to extract collagen macro/supramolecular structural aspects and found significant differences between normal and healthy tissues. We also present a new theoretical approach to phasematching in biological tissues as an additional characterization method. Collectively, this structural information provides insight into disease etiology.

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Paul J. Campagnola, Darian James, Emily Shelton, Nathan Sandbo, and Manish Patankar "High resolution imaging of collagen architecture alterations in ovarian cancer and idiopathic pulmonary fibrosis", Proc. SPIE PC12228, Ultrafast Nonlinear Imaging and Spectroscopy X, PC122280B (3 October 2022); https://doi.org/10.1117/12.2636163

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KEYWORDS

Collagen

Ovarian cancer

Pulmonary fibrosis

Image resolution

Tissues

Second-harmonic generation

Diagnostics

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