Effects of formalin fixation on ex vivo confocal microscopy imaging

Julia Kahn; Ucalene Harris; Anna Vaeth; Anthony Rossi; Manu Jain

doi:10.1117/12.2651961

17 March 2023 Effects of formalin fixation on ex vivo confocal microscopy imaging

Julia Kahn, Ucalene Harris, Anna Vaeth, Anthony Rossi, Manu Jain

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Proceedings Volume PC12352, Photonics in Dermatology and Plastic Surgery 2023; PC1235202 (2023) https://doi.org/10.1117/12.2651961
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Ex vivo confocal microscopy (EVCM) images tissues rapidly at near histological resolution without the need for histological processing. Other health institutes can benefit by sending their tissues to confocal experts in formalin, a readily available media that preserves tissue integrity. Fresh tissues imaged with an EVCM device at various timepoints after being put in formalin exhibited easier tissue flattening, improved visualization of the epidermis, reduced tissue movement (due to fat fixation), improved image contrast, and lack of photobleaching (due to dye fixation). Normal skin structures and tumors were readily identified at all TPs by an expert in real-time.

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Julia Kahn, Ucalene Harris, Anna Vaeth, Anthony Rossi, and Manu Jain "Effects of formalin fixation on ex vivo confocal microscopy imaging", Proc. SPIE PC12352, Photonics in Dermatology and Plastic Surgery 2023, PC1235202 (17 March 2023); https://doi.org/10.1117/12.2651961

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KEYWORDS

Tissues

Confocal microscopy

Skin

Tumors

Biopsy

Image processing

Image resolution

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