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Tissue-mimicking phantoms are vital for the calibration of imaging systems. Blood oxygenation is an important physiological parameter that spectral imaging devices can measure, motivating the need for blood phantoms with variable oxygenation. We present a soft lithography method for fabricating blood flow phantoms that enable control of channel size and blood oxygenation. Channel size was varied during the fabrication process. Oxygenation was varied by chemical oxygenation and deoxygenation of horse blood flowing in the phantom. The spectral properties of the phantoms were evaluated using a narrowband nailfold capillaroscopy system. The resulting phantoms yield a flexible approach for spectral imaging calibration.
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Michaela Taylor-Williams, Graham Spicer, Sarah E. Bohndiek, "Variable oxygenation blood flow phantoms for calibration of spectral imaging techniques," Proc. SPIE PC12363, Multiscale Imaging and Spectroscopy IV, PC123630N (17 March 2023); https://doi.org/10.1117/12.2649809