Dynamic full-field optical coherence microscopy of early mammalian oocytes

Seweryn Morawiec; Patrycjusz Stremplewski; Anna Ajduk; Brendan F. Kennedy; Maciej Szkulmowski

doi:10.1117/12.2649882

17 March 2023 Dynamic full-field optical coherence microscopy of early mammalian oocytes

Seweryn Morawiec, Patrycjusz Stremplewski, Anna Ajduk, Brendan F. Kennedy, Maciej Szkulmowski

Proceedings Volume PC12363, Multiscale Imaging and Spectroscopy IV; PC123630P (2023) https://doi.org/10.1117/12.2649882
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Optical coherence microscopy (OCM) facilitates imaging of biological processes in living cells with no need for fluorescence labeling and at power levels that do not harm the cells. In this paper, we present a compact full-field OCM system coupled with a commercial fluorescence microscope that allows for label-free imaging of living cells with high lateral and axial resolution. The dynamic imaging allows for the internal cell structures to be characterized based on their light scattering potential and motion dynamics. Additionally, we demonstrate that the internal motion of the cytoplasm effectively reduces the speckle noise resulting in high-contrast images.

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Seweryn Morawiec, Patrycjusz Stremplewski, Anna Ajduk, Brendan F. Kennedy, and Maciej Szkulmowski "Dynamic full-field optical coherence microscopy of early mammalian oocytes", Proc. SPIE PC12363, Multiscale Imaging and Spectroscopy IV, PC123630P (17 March 2023); https://doi.org/10.1117/12.2649882

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KEYWORDS

Optical coherence microscopy

Luminescence

Mirrors

Modulation

Phase shift keying

Phase shifts

Spatial resolution

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