All-optical neurophysiology using superfast polarization-sensitive off-axis full-field optical coherence microscopy (SPoOF OCM) at kilohertz frame rates

Rishyashring R. Iyer; Carlos A. Renteria; Jiho Kahng; Stephen A. Boppart

doi:10.1117/12.2649258

17 March 2023 All-optical neurophysiology using superfast polarization-sensitive off-axis full-field optical coherence microscopy (SPoOF OCM) at kilohertz frame rates

Rishyashring R. Iyer, Carlos A. Renteria, Jiho Kahng, Stephen A. Boppart

Author Affiliations +

Proceedings Volume PC12365, Neural Imaging and Sensing 2023; PC123650F (2023) https://doi.org/10.1117/12.2649258
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

One of the challenges in indirect measurements of electrical activity is its representation as biologically-relevant features. Non-invasive techniques for controlling neural activity, such as optogenetics with simultaneous optical imaging, have emerged as powerful and versatile tools. We demonstrated Superfast Polarization-sensitive Off-axis Full-field (SPoOF) OCM to image changes to both the optical phase and birefringence from the electrical activity of neurons at cellular resolution for an entire network at a millisecond scale. Here, we demonstrate all-optical neurophysiology with SPoOF OCM and optical excitation as a non-invasive versatile technique for studying neural circuits at high throughput, and a method to convert optical metrics to biologically relevant electrical features.

Conference Presentation

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Rishyashring R. Iyer, Carlos A. Renteria, Jiho Kahng, and Stephen A. Boppart "All-optical neurophysiology using superfast polarization-sensitive off-axis full-field optical coherence microscopy (SPoOF OCM) at kilohertz frame rates", Proc. SPIE PC12365, Neural Imaging and Sensing 2023, PC123650F (17 March 2023); https://doi.org/10.1117/12.2649258

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