Presentation
5 October 2023 Optical tweezers assessment of local ECM mechanical heterogeneity during capillary morphogenesis
Michelle Lanterman, Irene Zhang, Sarah Eldeen, Ulysses Castillo, Andrew Putnam, Elliot Botvinick
Author Affiliations +
Abstract
ECM stiffness is a potential instructive cue during capillary morphogenesis. Bulk measurements have often been used to investigate matrix stiffness as a cellular cue. However, local peri-cellular stiffness can be heterogeneous and vary greatly from measured bulk properties spatially and temporally. Here, we utilize bulk rheology and optical tweezers active microrheology (AMR) to investigate the dynamic mechanical crosstalk between cells and the surrounding matrix during capillary morphogenesis across length scales with various supporting stromal cells. We will present our current findings focusing on the discrepancies between bulk and microrheological distributions of measured stiffness across different stromal cell types.
Conference Presentation
© (2023) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Michelle Lanterman, Irene Zhang, Sarah Eldeen, Ulysses Castillo, Andrew Putnam, and Elliot Botvinick "Optical tweezers assessment of local ECM mechanical heterogeneity during capillary morphogenesis", Proc. SPIE PC12649, Optical Trapping and Optical Micromanipulation XX, PC126490T (5 October 2023); https://doi.org/10.1117/12.2681249
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KEYWORDS
Capillaries

Optical tweezers

Matrices

Bone

Confocal microscopy

Crosstalk

Hydrogels

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