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13 March 2024 Quantitative fluorescence imaging of drug distribution and therapeutic response at single-cell resolution
Lei G. Wang, Antonio Montano, Nathan McMahon, Allison Solanki, Kimberley Samkoe, Kenneth Tichauer, Summer Gibbs
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Proceedings Volume PC12821, Visualizing and Quantifying Drug Distribution in Tissue VIII; PC128210D (2024) https://doi.org/10.1117/12.3003598
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Quantifying intracellular drug target validation and target engagement has presented significant challenges, as drugs tend to accumulate non-specifically due to variations in drug affinity, biodistribution, pharmacokinetics, and metabolism. We have developed a ratiometric imaging approach, termed intracellular Paired Agent Imaging (iPAI), for estimating the concentration of protein receptors. This innovative technique holds great potential for revitalizing quantitative intracellular protein receptor imaging, offering a potential solution to quantify the availability of intracellular drug targets. Our approach involves the utilization of fluorophore-labeled small molecule therapeutics as imaging agents. We show it is now possible to use small molecule fluorophore labeled therapeutic inhibitors to visualize their targeted proteins intracellularly, providing a chemical tool kit to generate maps of bound and unbound inhibitors at the single-cell level both in vitro and ex vivo. iPAI platform provides a powerful new opportunity to study numerous other drugs in cells and tissues, resulting in a comprehensive spatial view of drug distribution and binding for improved personalized therapy.
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Lei G. Wang, Antonio Montano, Nathan McMahon, Allison Solanki, Kimberley Samkoe, Kenneth Tichauer, and Summer Gibbs "Quantitative fluorescence imaging of drug distribution and therapeutic response at single-cell resolution", Proc. SPIE PC12821, Visualizing and Quantifying Drug Distribution in Tissue VIII, PC128210D (13 March 2024); https://doi.org/10.1117/12.3003598
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KEYWORDS
Fluorophores
Tissues
Fluorescence imaging
Therapeutics
Biological imaging
Molecules
Dyes
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