Multimodal imaging combining VIS-OCT and phosphorescence lifetime SLO for comprehensive vascular structure and function in the mouse retina

Stephanie L. Nolen; Zhongqiang Li; Jingyu Wang; Ji Yi

doi:10.1117/12.3001132

13 March 2024 Multimodal imaging combining VIS-OCT and phosphorescence lifetime SLO for comprehensive vascular structure and function in the mouse retina

Stephanie L. Nolen, Zhongqiang Li, Jingyu Wang, Ji Yi

Author Affiliations +

Proceedings Volume PC12824, Ophthalmic Technologies XXXIV; PC1282403 (2024) https://doi.org/10.1117/12.3001132
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Oxygen partial pressure (pO2) has been measured in large retinal vessels using oxygen-dependent quenching of phosphorescent probe lifetimes, but existing systems face resolution or speed limitations due to camera-based or two-photon imaging. We developed a multimodal scanning laser ophthalmoscopy (SLO) and visible light optical coherence tomography (VIS-OCT) device to image murine retinae with balanced speed and resolution and capture additional vascular structure and function information. Phosphorescence intensity images using Oxyphor 2P demonstrated improved resolution, revealing smaller vascular structures, and improved throughput (~200 µs/pixel). VIS-OCT produced high resolution structural volumes and demonstrated doppler capabilities with alternating artery and vein phase shifts.

Conference Presentation

Citation Download Citation

Stephanie L. Nolen, Zhongqiang Li, Jingyu Wang, and Ji Yi "Multimodal imaging combining VIS-OCT and phosphorescence lifetime SLO for comprehensive vascular structure and function in the mouse retina", Proc. SPIE PC12824, Ophthalmic Technologies XXXIV, PC1282403 (13 March 2024); https://doi.org/10.1117/12.3001132

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